||Characterization of the 'Pseudomonas aeruginosa recA' Analog and Its Protein Product: 'rec-102' is a Mutant Allele of the 'P. aeruginosa' PAO 'recA' Gene.
Kokjohn, T. A. ;
Miller, R. V. ;
||Stritch School of Medicine, Maywood, IL. Dept. of Biochemistry and Biophysics.;Environmental Research Lab., Gulf Breeze, FL.
Pseudomonas aeruginosa ;
Escherichia coli ;
Deoxyribonucleic acids ;
Genetic intervention ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Cloned was a 2.3 kilobase pair fragment of the Pseudomonas aeruginosa PAO chromosome which is capable of complementing recA mutations of Escherichia coli. The recA-complementing activity was further localized to a 1.5 kilobase pair PvuII-HindIII fragment. The direction of transcription was determined. Southern analysis under conditions of high stringency indicated that DNA sequence homology is shared by the E. coli recA gene and the P. aeruginosa recA analogue. The cloned recA analogue was shown to restore resistance to methyl methane-sulfonate, nitrofurantoin and ultraviolet irradiation to E coli recA mutants. (Copyright (c) 1987, American Society for Microbiology.)