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RECORD NUMBER: 20 OF 395

OLS Field Name OLS Field Data
Main Title Application of DNA Probes to Analysis of Bacteriophage Distribution Patterns in the Environment.
Author Ogunseitan, O. A. ; Sayler, G. S. ; Miller, R. V. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Tennessee Univ., Knoxville. ;Oklahoma State Univ., Stillwater.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/388;
Stock Number PB93-121200
Additional Subjects DNA probes ; Bacteriophages ; Aquatic microbiology ; Soil microbiology ; Pseudomonas aeruginosa ; Viral genes ; Genetic transduction ; Nucleic acid hybridization ; Agar gel electrophoresis ; Autoradiography ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB93-121200 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/08/1993
Collation 9p
Abstract
Radiolabeled bacteriophage DNA probes have been used in the study to determine the distribution of Pseudomonas aeruginosa-infecting bacteriophages in natural samples of lake water, sediment, soil, and sewage. The sensitivity of detection of bacteriophage with the DNA probes was between 100 and 1,000 PFU and ten to the sixth power to ten to the seventh power CFU of lysogenized bacteria detectable with a homologous phage DNA probe. Analyses of environmental samples suggest that up to 40% of P. aeruginosa in natural ecosystems contain DNA sequences homologous to phage genomes. By using different bacteriophage DNA probes, the diversity of the bacteriophage population in sewage was estimated to be higher than that in other natural samples. The indication that transducing phages and prophages are widely distributed in the Pseudomonas populations investigated has considerable implications for the frequency of natural gene transfer by transduction and of lysogenic conversion of host bacteria in natural ecosystems. (Copyright (c) 1992, American Society for Microbiology.)