Record Display for the EPA National Library Catalog

RECORD NUMBER: 149 OF 395

OLS Field Name OLS Field Data
Main Title Extraction and Purification of Microbial DNA from Sediments.
Author Ogram, A. ; Sayler, G. S. ; Barkay, T. ;
CORP Author Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA-R-812488; EPA/600/J-87/314;
Stock Number PB88-196316
Additional Subjects Deoxyribonucleic acids ; Sediments ; Purification ; Extraction ; Chromatography ; Cells(Biology) ; Bacteria ; Centrifuging ; Microbiology ; Microorganisms ; Soils ; Reprints ; DNA
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB88-196316 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/04/1988
Collation 13p
Abstract
A new method for the isolation of intracellular and extracellular DNA from a range of sediment types has been developed. The method is based upon the direct lysis of cells in the sediment, extraction of released DNA from the sediments and its subsequent purification by CsC1-EtBr gradient centrifugation and/or hydroxyapatite chromatography. Yields of 26 micrograms intracellular DNA and 1 microgram extracellular DNA have been obtained per gram of sediment. (Copyright (c) Elsevier Science Publishers B.V. (Biomedical Division).)