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RECORD NUMBER: 136 OF 395

OLS Field Name OLS Field Data
Main Title Evaluation of DNA Damage in the Oral Mucosa of Tobacco Users and Non-Users by (32)P-Adduct Assay.
Author Chacko, M. ; Gupta, R. C. ;
CORP Author Baylor Univ., Houston, TX. Coll. of Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/468;
Stock Number PB90-147737
Additional Subjects Tobacco ; Carcinogens ; Evaluation ; Comparison ; Smoking ; Reprints ; DNA adducts ; Mouth mucosa ; Tobacco use disorder ; Smokeless tobacco
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-147737 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/15/1990
Collation 7p
Abstract
Tobacco and its combustion products contain several known or potential human carcinogens and studies are not beginning to emerge for detecting DNA and protein adducts in tobacco users. A highly sensitive (32)P-adduct assay, capable of measuring a wide spectra of aromatic and/or hydrophobic DNA adducts, was used to evaluate tobacco use-related adducts in human oral mucosal cells. The chromatographic behavior of the oral mucosal DNA adducts by selective PE1-cellulose TLC and their extractability in 1-butanol suggest that they have an aromatic and/or hydrophobic moiety and two of the major adducts have been tentatively assigned as aromatic amine derivatives.