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RECORD NUMBER: 123 OF 395

OLS Field Name OLS Field Data
Main Title Dynamic Interactions of 'Pseudomonas aeruginosa' and Bacteriophages in Lake Water.
Author Ogunseitan, O. A. ; Sayler, G. S. ; Miller, R. V. ;
CORP Author Tennessee Univ., Knoxville. Center for Environmental Biotechnology. ;Stritch School of Medicine, Maywood, IL. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA-R8115234; EPA/600/J-90/369;
Stock Number PB91-163832
Additional Subjects Bacteriophages ; Water microbiology ; Pseudomonas aeruginosa ; Base sequence ; Lakes ; Colony formation ; Nucleic acid hybridization ; Electron microscopy ; Viral DNA ; Agar gel electrophoresis ; Reprints ;
Holdings
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Status
NTIS  PB91-163832 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/13/1991
Collation 17p
Abstract
The persistence and interaction between newly isolated strains of Pseudomonas aeruginosa and resident bacteriophages indigenous to a freshwater environment was monitored over 45 days in lake water microcosms. The interaction between susceptible and resistant bacteria with pure phage (UT1) particles or a mixed phage population (M1) was investigated by following temporal changes in host density, phage-to-bacteria ratio (PBR), and the appearance of apparent prophage carriers within the host population. Decay rates of the phage (UT1) ranged from 0.054/hour in natural water to 0.027/hour in natural water to 0.027/hour in filtered lake water. About 45% of sensitive bacteria incubated with phase UT1 were pseudolysogenic within 12 hours of incubation in natural lake water. Phage UT1 appeared to stabilize the density of host bacteria in lake water at a level of 10 to the fourth power colony-forming units (cfu)/ml. Bacterial coexistence with the mixed phage (M1) population resulted in an oscillating equilibrium with the PBR stabilizing at about 3. (Copyright (c) Springer-Verlag New York, Inc. 1990.)