||Growth of an Adherent Mixed Microbial Culture in a Substrate Limited Single State Chemostat.
Pfiste, Robert M. ;
||Ohio State Univ., Columbus. Water Resources Center.
||391X; OWRR-A-021-OHIO; 02661,; A-021-OHIO(1)
Aquatic microbiology ;
Culture media ;
Biological productivity ;
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The objective was to develop a continuous culture system with two bacterial cultures, one of which was adherent to the wall of the chemostat. By developing such a laboratory model, microbial interactions in certain aquatic ecosystems (e.g. river sediments) can be further understood. A steady-state was established between a Chromobacterium lividum and Pseudomonas sp. at a dilution rate of 0.27/hr when the growth limiting substrate was citrate. During both pure and mixed continuous culture studies the C. lividum adhered to the wall of the chemostat and the Pseudomonas showed no such tendency visually. This system offers a convenient model for studying the importance of bacterial adherence to certain aquatic ecosystems such as river sediments and sewage treatment. (Modified author abstract)