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RECORD NUMBER: 3 OF 3

OLS Field Name OLS Field Data
Main Title Immunofluorescence Detection of 'Cryptosporidium' Oocysts in Fecal Smears.
Author Stibbs, H. H. ; Ongerth, J. E. ;
CORP Author Washington Univ., Seattle. School of Public Health and Community Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/299;
Stock Number PB87-175931
Additional Subjects Para sitology ; Feces ; Coccidioides ; Methyl sulfoxide ; Benzophenones ; Fluorescence ; Biological stains ; Cattle ; Primates ; Reprints ; Crytosporidium ; Coccidia ; Macaca nemestrina ; Rosaniline dyes ; Dimethyl sulfoxide
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB87-175931 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 8p
Abstract
An indirect fluorescent antibody (IFA) procedure was developed for the detection of Cryptosporidium sp. oocysts in human, nonhuman primate, and bovine fecal smears. The procedure, which takes about 90 min to perform, involves the use of a rabbit antiserum against Cryptosporidium oocysts isolated from dairy cattle. Cross-specificity testing of the IFA method revealed no reactivity with yeasts, various amoebae, Giardia lamblia, Chilomastix sp., or Blastocystis sp. and only very weak cross-reactivity with coccidian oocysts of other genera. IFA detection of oocysts in human and nonhuman primate fecal smears was far more sensitive than was dimethyl sulfoxide-carbolfuchsin staining. Moreover, IFA detection was comparable in sensitivity to auramine O staining with samples of high oocyst concentration and somewhat more sensitive than auramine O with sample containing relatively few oocysts. The IFA procedure may be useful in the clinical diagnosis of human and animal cryptosporidiosis and also in the detection of oocysts in environmental samples. (Copyright (c) 1986, American Society for Microbiology.)