||Mutagenicity in Salmonella of Hazardous Wastes and Urine from Rats Fed These Wastes.
DeMarini, D. M. ;
Inmon, J. P. ;
Simmons, J. E. ;
Berman, E. ;
Pasley, T. C. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Industrial wastes ;
Hazardous materials ;
Chromosome abnormalities ;
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Fifteen hazardous industrial waste samples were evaluated for mutagenicity in the Salmonella plate-incorporation assay using strains TA98 and TA100 in the presence and absence of Aroclor 1254-induced rat liver S9. Dichloromethane/methanol extracts of the crude wastes also were evalauted. Seven of the crude wastes were mutagenic, but only 2 of the extract of these 7 wastes were mutagenic; extracts of 2 additional wastes also were mutagenic. In addition, 10 of the crude wastes were administered by gavage to F-344 rats, and 24-H urine samples were collected. Of the 10 raw urines evaluated, 3 were mutagenic in strain TA98 in the presence of S9 and B-glucuronidase. To the authors' knowledge, this is the first report of the mutagenicity of urine from rodents exposed to hazardous wastes. Based on the present results, the use of only strain TA98 in the presence of S9 might be adequate for general screening of hazardous wastes or waste extracts for genotoxicity.