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RECORD NUMBER: 37 OF 53

OLS Field Name OLS Field Data
Main Title Monochromosomal Hybrid Cell Assay for Evaluating the Genotoxicity of Environmental Chemicals.
Author Sandhu, S. S. ; Gudi, R. D. ; Athwal, R. S. ;
CORP Author New Jersey Medical School, Newark.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1988
Report Number EPA-R-812207; EPA/600/D-88/152;
Stock Number PB88-238134
Additional Subjects Toxicity ; Chromosome abnormalities ; Methodology ; Bacterial genes ; Health effects ; Toxic substances ; Cytogenetics ; Hybrid cells ; Genetic engineering ; Human chromosomes ; Aneuploidy
Holdings
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Status
NTIS  PB88-238134 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/29/1988
Collation 25p
Abstract
The development and utilization of a monochromosomal hybrid cell assay for detecting aneuploidy and chromosomal aberrations are described. The monochromosomal hybrid cell lines were produced by a two-step process involving transfer of a marker bacterial gene to a human chromosome and then by integration of that human chromosome into a mouse complement of chromosomes through microcell fusion. For chemical induced aneuploidy, the segregation of a single human chromosome among cause chromosomes is used as a cytogenetic marker. The genetic assay for aneuploidy is based on the ability of cells to grow in a medium that selects for the loss of the human chromosome. The assay for clastogenicity is based on survival of cells after treatment with chemicals in medium that selects for retention of the human chromosome but loss of its segment containing DT locus. The assays greatly simplify the detection of chromosomal aberrations induced by environmental factors at low-dose levels.