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RECORD NUMBER: 36 OF 53

OLS Field Name OLS Field Data
Main Title Micronucleus, Chromosome Aberration, and Small-Colony TK Mutant Analysis to Quantitate Chromosomal Damage in L5178Y Mouse Lymphoma Cells.
Author Doerr, C. L. ; Harrington-Brock, K. ; Moore, M. M. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1989
Year Published 1989
Report Number EPA-68-02-4456; EPA/600/J-89/047;
Stock Number PB90-100546
Additional Subjects Chromosome abnormalities ; Lymphomas ; Mutation ; Cells(Biology) ; Mice ; Actinomycin ; Methylsulfoxide ; Methylcholanthrene ; Reprints ; Micronucleus test ; Toxic substances ; Adriamycin ; Bleomycin ; Methylmethacrylates ; Proflavine ; Hazardous waste ; n-AMSA
Holdings
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Status
NTIS  PB90-100546 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 16p
Abstract
The micronucleus test has been advocated as a rapid and easy alternative to aberration analysis. In testing the hypothesis that the small-colony thymidine kinase deficient mutants of L5178Y/TK+/- -3.7.2C mouse lymphoma cells represent an estimate of the clastogenicity of test chemicals, gross aberration analysis has been performed. The present study was initiated to determine if the cytokinesis block method of micronucleus analysis could be performed in mouse lymphoma cells. In this study, 12 compounds having varying clastogenic potencies were evaluated. Three endpoints, the number of metaphases with aberrations, number of binucleates with micronuclei, and small-colony TK mutant frequency, were evaluated. All three endpoints, the number of metaphases with aberrations, number of binucleates with micronuclei, and small-colony TK mutant frequency, were evaluated. All three endpoints can be used to estimate the clastogenicity of test agents. As would be expected, the three endpoints vary in the relative magnitude of the quantitated response. This difference likely results from the types of clastogenic damage detected by each endpoint. (Copyright (c) 1989 Elsevier Science Publishers B.V. (Biomedical Division.)