Abstract |
BPS-24 was submitted for testing and evaluation of clastogenic potential. Chromosomal aberrations assays were performed with duplicate, Chinese hamster ovary (CHO) cell cultures. This study was conducted incorporating 2 independent tests. Dimethylsulphoxide (DMSO) was the vehicle and cyclophosphamide and methyl methanesulphonate were the positive controls used in both experiments. Both tests were conducted in both the presence and absence of a post-mitochondrial supernatant fraction obtained from the livers of adult, male rats treated with Aroclor 1254 (S9) and an NADPH-generating system. Cultures, established approximately 20 hours before testing, were treated for 6 h in the presence or 22 hours in the absence of 59 mix. Cultures were harvested at 24 h (Test 1 and 2) or 48 h (Test 2) post treatment. BPS-24 was toxic to Chinese hamster ovary cells in vitro when tested to the maximum permitted concentration of 5000 micro g. ml(sup -1) in Test 1. Toxicity was noted in cultures treated with 313-5000 micro g.ml(sup -1) in the presence and 625-5000 micro g.ml(sup -1) in the absence of S9 mix. In Test 2, cultures were tested with 78-625 micro g. ml(sup -1) in both the presence and absence of S9 mix. Toxicity was noted in cultures treated with 313-625 micr g.ml(sup -1) in the presence and 234-625 micro g.ml(sup -1) in the absence of S9 mix. |