Record Display for the EPA National Library Catalog

RECORD NUMBER: 32 OF 143

OLS Field Name OLS Field Data
Main Title Critical Study of the Manometric Assay of Cholinesterase in Rat Blood.
Author Witter., Robert F. ;
CORP Author Communicable Disease Center, Atlanta, Ga.
Year Published 1961
Stock Number PB-275 929
Additional Subjects Cholinesterase ; Chemical analysis ; Assaying ; Blood chemical analysis ; Manometers ; Enzymes ; Rats ; Laboratory animals ; Tables(Data) ; Esterases ; Hydrolases ; Age ; Reprints
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB-275 929 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 11p
Abstract
A study was made of the conditions necessary for the quantitative assay of the erythrocyte and plasma cholinesterases of the rat by the Warburg manometric method. Butyrylcholine, acetyl-beta-methylcholine, and acetylcholine were tested as substrates, but only acetylcholine proved satisfactory. The level of the red cell cholinesterase did not change with age of the rat. The erythrocytes were found to contain an esterase which hydrolyzes butyrylcholine. In confirmation of other workers, it was found that the plasma cholinesterase levels of female rats increase with age and are higher than those of male rats of all ages and that the plasma of male animals contains an approximately equal mixture of true and pseudocholinesterase whereas that of female rats contains mainly pseudocholinesterase.