Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Methods Used in Determination of Cholinesterase Activity.
Author Mineau., P. ;
CORP Author Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology International, Inc., Corvallis, OR. ;Clemson Univ., SC. Inst. of Wildlife and Environmental Toxicology.
Publisher c1991
Year Published 1991
Report Number EPA/600/A-92/078;
Stock Number PB92-179035
Additional Subjects Cholinesterase ; Toxicology ; Cholinesterase inhibitors ; Test methods ; pH ; Temperature ; Cations ; Substrate specificity ; Wildlife ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-179035 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/22/1992
Collation 38p
The chapter provides an overview and historical perspective of the many available methods for cholinesterase (ChE) activity determination. Due to the almost universal use of the spectrophotometric assay in wildlife toxicology, the remainder of the chapter focuses on this technique. Sources of variability in the assay are discussed, including assay temperature, instrumentation, degree of solubilization of membrane-bound enzyme, and lipid concentration in serum samples. Methods to control these sources of error are offered. Consideration is given to methods of differentiating the activity of the various esterases (for example, acetylcholinesterase and butyrylcholinesterase). A brief review is provided of commercial kits available for field and/or laboratory testing of samples for ChE activity. The chapter includes a discussion of future innovations and refinements of the ChE assay, including the use of reactivation techniques and adaptation of the assay for use with 96-well microtitration plates. (Copyright (c) 1991 Elsevier Science Publishers B.V.)