Record Display for the EPA National Library Catalog

RECORD NUMBER: 31 OF 97

OLS Field Name OLS Field Data
Main Title Enzyme-Based Detection of Chlorinated Hydrocarbons.
Author Offenhartz, B. H. ; Lefko, J. L. ;
CORP Author B and M Technological Services, Inc., Boston, MA.;Municipal Environmental Research Lab., Cincinnati, OH.
Year Published 1984
Report Number EPA-68-03-3113; EPA-600/D-84-003;
Stock Number PB84-125004
Additional Subjects Cholinesterase inhibitors ; Chlorine organic compounds ; Chemical analysis ; Environmental surveys ; Hydrocarbons ; Catalysis ; Pesticides ; Aldrin ; Immobilized enzymes ; Toxaphene ; Aroclor 1242 ; Phenol/pentachloro
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB84-125004 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 19p
Abstract
Recent advances in immobilized enzyme-based analytical methods, e.g., the cholinesterase-based water monitor 'CAM' (cholinesterase antagonsist monitor), have proved useful in the detection of organophosphate and carbamate pesticides. This work has now been extended to the detection of five high molecular weight chlorinated hydrocarbons at the parts-per-million (ppm) level in a water/methanol matrix. In the new method being developed, the lactate dehydrogenase (LDH) catalyzed reaction: pyruvate + NADH + H(+) yields (LDH) (inhibitor) lactate + NAD(+1) is used to detect the presence of chlorinated hydrocarbons, which reduce the rate of the reaction by inhibiting the enzyme. To date, the method has proved effective in detecting aldrin, toxaphene, Aroclor 1242, DDT, and pentachlorophenol. Nonchlorinated analogs, e.g. phenols, are detected only at orders of magnitude higher concentrations. The method has not yet been evaluated on environmental samples.