Main Title |
Polymeric Beta-Hydroxyalkanoates from Environmental Samples and 'Bacillus megaterium'. |
Author |
Findlay, Robert H. ;
White, David C. ;
|
CORP Author |
Florida State Univ., Tallahassee.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.;National Oceanic and Atmospheric Administration, Rockville, MD. Office of Sea Grant. |
Year Published |
1983 |
Report Number |
EPA-R-807321 ;NSF-OCE80-19757; EPA-600/J-83-016; |
Stock Number |
PB83-223727 |
Additional Subjects |
Bacteria ;
Chemical analysis ;
Sediments ;
Extraction ;
Oxygen organic compounds ;
Gas chromatography ;
Sampling ;
Purification ;
Nutrients ;
Metabolism ;
Fatty acids ;
Reprints ;
Poly(butyric acid/(hydroxy-ester)) ;
Bacillus megaterium
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB83-223727 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The procaryotic endogenous storage polymer known as poly-beta-hydroxybutyrate is actually a mixed polymer of short-chain beta-hydroxy fatty acids. A method for the quantitative recovery of this mixed polymer, called poly-beta-hydroxyalkanoate (PHA), with analysis by capillary gas-liquid chromatography, showed the presence of at least 11 short-chain beta-hydroxy acids in polymers extracted from marine sediments. Polymers extracted from Bacillus megaterium monocultures were also a complex mixture of beta-hydroxy acids with chain lengths between four and eight carbons. Lyophilized sediments were extracted in a modified Soxhlet extractor, and the polymer was purified with ethanol and diethyl ether washes. The purified polymer was treated with ethanol-chloroform-hydrochloric acid (8.5:2.5:1) for 4 h at 100 C, a treatment which resulted in the formation of the ethyl esters of the constituent beta-hydroxy acids. |