Record Display for the EPA National Library Catalog

RECORD NUMBER: 5 OF 10

OLS Field Name OLS Field Data
Main Title Method 1605 : Aeromonas in finished by membrane filtration, September 2000-draft.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher U.S. Environmental Protection Agency, Office of Water,
Year Published 2000
Report Number EPA/821/R-00/026
Stock Number PB2001-101413
OCLC Number 49863225
Additional Subjects Membrane filtration ; Bacteria ; Water sampling ; Drinking water ; Filtration ; Growth(Biology) ; Water filters ; Water analysis ; Pore size ; Dextrins ; Incubation ; Agar ; Fermentation ; Carbohydrates ; Acidity ; Chemical indicators ; Colors ; Cytochrome oxidase ; Trehalose ; Water pollution effects ; Surface waters ; Contamination ; Quality control ; Aeromonas ; Ampicillin ; Bromothymol blue ; Oxidase tests ; Cytochrome C
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=20012A7W.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EJBD ARCHIVE EPA 821-R-00-026 Headquarters Library/Washington,DC 10/11/2011
EJBD  EPA 821-R-00-026 c.1 Headquarters Library/Washington,DC 01/08/2014
ESAD  EPA 821-R-00-026 Region 10 Library/Seattle,WA 05/24/2002
NTIS  PB2001-101413 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 28 p. : ill. ; 28 cm.
Abstract
The method provides a direct count of Aeromonas in water based on the development of yellow colonies on the surface of the membrane filter using a selective media for Aeromonas species. A water sample is filtered through 0.45 micrometer membrane filter. The filter is placed on ampicillin-dextrin agar and incubated at 35 C +/- 0.5 C for 22 to 26 hours. This medium uses 10-milligram/liter ampicillin to inhibit non-Aeromonas species, while allowing Aeromonas to grow. The medium uses dextrin as a fermentable carbohydrate, and bromothymol blue as an indicator of acidity produced by the fermentation of dextrin. Yellow presumptively identified colonies are counted and confirmed by testing for the presence of cytochrome c (oxidase test) and the ability to ferment trehalose. The membrane filtration procedure provides a direct count of culturable Aeromonas in water samples that is based on the bacterial growth on the surface of the membrane.
Notes
Cover title. "September 2000." "EPA 821-R-00-026."