Record Display for the EPA National Library Catalog

RECORD NUMBER: 9 OF 23

OLS Field Name OLS Field Data
Main Title Improved Method for the Determination of Aryl-4-hydroxylase Activity.
Author Lindblad, William J. ; Jackim, Eugene ;
CORP Author Environmental Research Lab., Narragansett, RI.
Year Published 1981
Report Number EPA-600/J-81-525 ;ERLN-J185;
Stock Number PB82-167230
Additional Subjects Filtration ; Separation ; Enzymes ; Revisions ; Toxicology ; Extraction ; Fluorescence ; Cellulose acetate ; Reprints ; Hydroxylases/aryl ; Tritium release assay ; Benzopyrenes
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB82-167230 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 3p
Abstract
An improved method for separation of tritiated benzo(a) pyrene from tritiated water in the aryl-4-hydroxylase assay is presented. Quantitative retention of benzo(a) pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modification agreed well with those determined using the standard charcoal column extraction. This modification greatly reduces the time required to perform this assay, and allows for the determination of enzyme activity in a large number of samples.