||Improved Method for the Determination of Aryl-4-hydroxylase Activity.
Lindblad, William J. ;
Jackim, Eugene ;
||Environmental Research Lab., Narragansett, RI.
Cellulose acetate ;
Tritium release assay ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
An improved method for separation of tritiated benzo(a) pyrene from tritiated water in the aryl-4-hydroxylase assay is presented. Quantitative retention of benzo(a) pyrene was obtained with mixed cellulose acetate and nitrate filters. Enzymatic activities obtained with this modification agreed well with those determined using the standard charcoal column extraction. This modification greatly reduces the time required to perform this assay, and allows for the determination of enzyme activity in a large number of samples.