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RECORD NUMBER: 13 OF 27

OLS Field Name OLS Field Data
Main Title Culture of animal cells : a manual of basic technique /
Author Freshney, R. Ian.
Publisher Wiley-Liss,
Year Published 2005
OCLC Number 58830118
ISBN 0471453293 9780471453291
Subjects Tissue culture--Laboratory manuals ; Cell culture--Laboratory manuals ; Cells, Cultured ; Culture Techniques ; Tissus (Histologie)--Culture--Manuels de laboratoire ; Cellules--Culture--Manuels de laboratoire
Internet Access
Description Access URL
http://www.loc.gov/catdir/enhancements/fy0626/2005281585-b.html
http://www.loc.gov/catdir/enhancements/fy0626/2005281585-d.html
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EOBM  QH585.2.F74 2005 NEIC Library/Denver,CO 02/09/2007
Edition 5th ed.
Collation 642 p. : ill. (some col.) ; 29 cm.
Notes
Includes bibliographical references (p. 581-626) and index.
Contents Notes
Introduction -- Training programs -- Biology of cultured cells -- Laboratory design and layout -- Equipment -- Aseptic technique -- Safety, bioethics, and validation -- Culture vessels and substrates -- Defined media and supplements -- Serum-free media -- Preparation and sterilization -- Primary culture -- Subculture and cell lines -- Cloning and selection -- Cell separation -- Characterization -- Differentiation -- Transformation and immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Culture of specific cell types -- Culture of tumor cells -- Organotypic culture -- Scale-up -- Specialized techniques -- Problem solving -- In conclusion.