Main Title |
Method for Recovering Viruses from River Water Solids. |
Author |
Berg, Gerald ;
Dahling, Daniel R. ;
|
CORP Author |
Environmental Monitoring and Support Lab.-Cincinnati, OH. Biological Methods Branch. |
Year Published |
1980 |
Report Number |
EPA-600/J-80-157 ;EMSL-191; |
Stock Number |
PB81-126476 |
Additional Subjects |
Viruses ;
Polioviruses ;
Recovery ;
Isolation ;
Rivers ;
pH ;
Cell cultures ;
Water ;
Reprints ;
Water pollution control
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB81-126476 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
6p |
Abstract |
Small numbers of virions (poliovirus 1) that had been adsorbed to river water solids were eluted by mixing the solids for 30 minutes with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures. With this method, 39 to 63% of the poliovirions that had been adsorbed onto the river water solids were recovered. |