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RECORD NUMBER: 8 OF 65

OLS Field Name OLS Field Data
Main Title Analysis of DNA-Carcinogens Adducts by Electrospray CZE-MS.
Author Barry, J. P. ; Vouros, P. ; Norwood, C. ;
CORP Author Northeastern Univ., Boston, MA. Barnett Inst.;Environmental Research Lab., Narragansett, RI.
Publisher 1994
Year Published 1994
Report Number EPA/600/A-94/260 ; ERLN-1632
Stock Number PB95-177325
Additional Subjects DNA adducts ; Carcinogens ; Benzo(a)pyrene ; Chemical analysis ; Neoplastic cell transformation ; Mutations ; Reprints ; Electrospray mass spctrometry ; Capillary zone electrophoresis
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB95-177325 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/26/1995
Collation 6p
Abstract
Polycyclic aromatic hydrocarbons are an important class of chemical carcinogen to which there is potentially extensive chronic human exposure. It is generally believed that an interaction of a reactive intermediate with cellular DNA is the initial step in the induction of mutations, neoplastic cell transformation and chemical carcinogenesis. Benzo(a)pyrene, the prototypic member of this class reacts with DNA through the intermediacy of a benzo(a)pyrene-7,8-dihydrodiol 9,10-epoxide metabolite (BPDE). There is considerable interest in examining these interactions. However, such research is hindered by the low levels of DNA adduct formation (typically 1 in ten to the fifth power to 1 in ten to the ninth power bases are modified by exposure). The paper reports on a preliminary investigation of the suitability of electrospray CZE-MS for characterizing benzo(a)pyrene adducts to DNA.