||Initial Submission: 24-Hour Exposure Clonal Syrian Golden Hamster Embryo (SHE) Cell Transformation Assay using Ortho-Toluidine with Cover Letter dated 04/30/1996.
||Proctor and Gamble Co., Cincinnati, OH. Ivorydale Technical Center.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Health effects ;
Toxic substances ;
Chronic toxicity ;
Cell transformation ;
In vitro ;
CAS No 636-21-5
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
A 24 hour exposure SHE cell transformation assay was performed to test the morphological transformation potential of ortho-Toluidine. The assay was performed according to the methodology outlined in the attached protocol. In the 24 hour SHE assay ortho-Toluidine gave significant (p<0.05) morphologicd transformation results, relative to the solvent control, at 4 of 5 tested concentrations. The trend test result for this assay was also significantly positive. According to our established criteria which states that either two positive doses or one positive dose and a positive trend test constitutes a positive SHE cell transformation assay result, ortho-Toluidine is considered positive in the 24 hour exposure SHE cell transformation assay.