Record Display for the EPA National Library Catalog

RECORD NUMBER: 42 OF 51

OLS Field Name OLS Field Data
Main Title Specificity of Cellular DNA-Binding Sites of Microbial Populations in a Florida Reservoir.
Author Paul, J. H. ; Pichard., S. L. ;
CORP Author University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/553;
Stock Number PB92-129618
Additional Subjects Aquatic microbiology ; Deoxyribonucleic acids ; Cell membrane ; Reservoirs ; Substrate specificity ; Florida ; Phosphates ; Fresh water ; Oligonucleotides ; Thymidine ; Thymine ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-129618 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 6p
Abstract
Substrate specificity of the DNA binding mechanism(s) of bacteria in a Florida Reservoir was investigated in short and long term uptake studies with radiolabelled DNA and unlabelled competitors. Thymine oligonucleotides ranging in size from 2 bp to 19-24 bp inhibited DNA binding in 20 min incubations by 43 to 77%. Deoxynucleoside monophosphates, thymidine, and thymine had little effect on short term DNA binding, although several of these compounds inhibited the uptake of radiolabel from DNA in 4 h incubations. RNA inhibited both short and long term radiolabelled DNA uptake equally as well as unlabelled DNA. Collectively these results indicate that aquatic bacteria posses a generalized nucleic acid uptake/binding mechanism specific for compounds containing phosphodiester bonds and capable of recognizing oligonucleotides as short as dinucleotides.