Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Individual and Combined Cytotoxic Effects of Cadmium, Copper, and Nickel on Brown Cells of 'Mercenaria mercenaria'.
Author Zaroogian, G. ; Anderson, S. ; Voyer, R. A. ;
CORP Author Environmental Research Lab., Narragansett, RI.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-93/308 ;ERLN-1340;
Stock Number PB93-229300
Additional Subjects Water pollution effects(Animals) ; Toxicity ; Cadmium ; Copper ; Nickel ; Cells(Biology) ; Cell survival ; Divalent cations ; In vitro analysis ; Lysosomes ; Reprints ; Mercenaria mercenaria ; Brown cells
Library Call Number Additional Info Location Last
NTIS  PB93-229300 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/22/1993
Collation 13p
An assay based on the lysosomal incorporation of neutral red dye by brown cells of Mercenaria mercenaria (Bivalvia) was used to measure the cytotoxicity of Cd(sup 2+), Cu(sup 2+), and Ni(sup 2+) singly and in combination. Cytotoxicity was a linear function of Cd(sup 2+) concentration 0.1-1.5 mM and Cu(sup 2+) concentration between 10 and 100 microM. Nickel was not cytotoxic at concentrations as high as 10 mM. The presence of Cu(sup 2+) lessened the cytotoxic effect of Cd(sup 2+). Ni(sup 2+) did not affect cytotoxicity in combination with either Cu(sup 2+) or Cd(sup 2+). Ni(sup 2+) inflated estimates of cell survival by the neutral red assay in this study. Cells exposed to Ni(sup 2+) yielded measured quantities of neutral red dye in excess of those measured in cells from control treatments. (Copyright (c) 1992 Academic Press, Inc.)