||Enzyme and Tissue Alterations in Fishes: A Measure of Water Quality.
Hinto, David E. ;
Kendal, M. W. ;
Koeni, J. C. ;
||Kentucky Water Resources Inst., Lexington.
||RR-68; DI-14-31-0001-3517 ;DI-14-31-0001-3217; OWRR-A-038-KY; 05540,; A-038-KY(3)
Water quality ;
Fresh water fishes ;
Mercury organic compounds ;
Indicator species ;
Water pollution effects(Animals) ;
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A variety of freshwater fishes were studied by light and electron microscopy, enzyme histochemical and biochemical methods. The objective was to determine normal structure and function in specific target organs and to compare these to altered states in aquatic pollution. The basic question, 'can fish tissues and enzymes serve as indicators of water quality,' was asked. Microscopic alteration in gill was indicative of copper toxicity at an exposure of 20 parts per billion. Gross and light microscopic alterations were indicative of a single exposure of channel catfish to 15 parts per million of methyl mercuric chloride (CH3HgCl). Microscopic and correlated biochemical study fingerprinted the alterations in cells at an exposure of 0.67 parts per million CH3HgCl. The developments of pathobiological autopsy techniques for the assessment of water quality is discussed.