||Multiple-Endpoint Mutagenesis with Chinese Hamster Ovary (CHO) Cells: Evaluation with Eight Carcinogenic and Non-Carcinogenic Compounds.
Hsie, A. W. ;
San Sebastian, J. R. ;
Perdue, S. W. ;
Schenley, R. L. ;
Winters, M. D. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Texas Univ. Medical Branch at Galveston. Dept. of Preventive Medicine and Community Health. ;Oak Ridge National Lab., TN. ;Pharmakon Research International, Inc., Waverly, PA.
Malignant neoplasms ;
Hypoxanthine phosphoribosyltransferase ;
Sister chromatid exchange ;
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Using Chinese hamster ovary (CHO) cells in culture, the authors have defined an assay, CHO/HGPRT, to quantify mutagen-induced cytotoxicity and mutations at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus. This assay permits elucidation of the structure-activity relationship and analysis of relative mutagenic potency of various classes of chemical mutagens. By expanding the CHO/HGPRT assay to include chromosome aberration and sister-chromatid exchange (SCE), the authors can analyze the interrelationships of these four distinct biological effects and compare each endpoint for its predictive power of human-level effect. (Copyright (c) 1987 by Hemisphere Publishing Corporation.)