||Respirometric multi-level biodegradation testing protocol and methodology for determining biodegradation kinetic data /
Tabak, Henry H. ;
Desai, S. ;
||Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Cincinnati Univ., OH. Dept. of Chemical and Nuclear Engineering.
|| U.S. Environmental Protection Agency, Office of Research and Development, Risk Reduction Engineering Laboratory,
Toxicity testing--Methodology. ;
Hazardous wastes--Biodegradation. ;
Organic compounds--Biodegradation. ;
Mechanics, Analytic. ;
Toxic substances ;
Soil contamination ;
Volatile organic compounds
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||29 pages : illustrations ; 28 cm
Electrolytic respirometry involving natural sewage, sludge and soil microbiota is becoming prominent in fate studies of priority pollutant and RCRA toxic organics to generate biodegradation/inhibition kinetics data. The paper discusses the experimental design and procedural steps for the respirometric biodegradation and toxicity testing approach for individual organics or specific industrial wastes at different concentration levels in a mineral salts medium. A developed multi-level protocol is presented for determination of the biodegradability, microbial acclimation to toxic substrates and first order kinetic parameters of biodegradation for estimation of the Monod kinetic parameter of toxic organic compounds, in order to correlate the extent and rate of biodegradation with a predictive model based on chemical properties and molecular structure of these compounds. Respirometric biodegradation/inhibition and biokinetic data are provided for representative RCRA alkyl, chloro- and nitro-benzenes, phenols and selected CERCLA leachate toxic organics. Data on the effects of the source of sludge biomass, temperature and concentration of microbial inoculum on the kinetics of biodegradation are also included.
"Henry H. Tabak, project officer." "Air & Waste Management Association, for presentation at the 83rd Annual Meeting & Exhibition, Pittsburgh, Pennsylvania, June 24-29, 1990." "Risk Reduction Engineering Laboratory, Office of Research and Development, U.S. Environmental Protection Agency."--Page i. Includes bibliographical references. "PB90-264003." "EPA/600/D-90/140." "90-21.6." Microfiche.