Record Display for the EPA National Library Catalog
RECORD NUMBER: 365 OF 1240
|OLS Field Name||OLS Field Data|
|Main Title||Dehydrogenation: A Previously Unreported Pathway of Lindane Metabolism in Mammals.|
|Author||Chadwick, Robert W. ; Chuang, Lucy T. ; Williams., Katherine ;|
|CORP Author||National Environmental Research Center, Research Triangle Park, N.C. Pesticides and Toxic Substances Effects Lab.|
|Report Number||found in PB-280 889|
|Stock Number||PB-280 894, found in PB-280 889|
|Additional Subjects||Pesticides ; Toxicology ; Insecticides ; Dehydrogenation ; Mammals ; In vitro analysis ; In vivo analysis ; Metabolism ; Chlorine organic compounds ; DDT ; Assaying ; Physiological effects ; Enzymes ; Liver ; Biochemistry ; Toxic substances ; Reprints ; Lindane ; Metabolites ; Cyclohexene/hexachloro|
The study presents evidence for the dehydrogenation of lindane by a hepatic microsomal mixed-function oxidase system. Preliminary investigation established that the incubation of lindane with rat liver homogenates produces a chlorinated, nonpolar compound identified as hexachlorocyclohexene. Differential centrifugation resulted in the sedimentation of most of the dehydrogenase activity in the microsomal fraction. Optimum in vitro assay conditions were established and it was found that the dehydrogenase system required molecular oxygen and reduced pyridine nucleotide coenzyme for maximum activity. Inhibition by SKF 525-A and CO suggested that the enzyme was cytochrome P-450 dependent. Lack of inhibition by cyanide indicated that the cytochrome b5 desaturase system was probably not involved. Pretreatment of rats with DDT, which stimulates lindane metabolism, also induced significantly higher dehydrogenase activity. Both the in vivo and in vitro metabolism of hexachlorocyclohexene produced previously identified lindane metabolites. The existence of a cytochrome P-450 dependent mixed-function oxidase which catalyzes the dehydrogenation of lindane has not previously been reported and may be of importance in the metabolism of other xenobiotics.
contained in PB-280 889 titled Journal Articles on Toxicology. Group 14.