Record Display for the EPA National Library Catalog

RECORD NUMBER: 365 OF 1240

OLS Field Name OLS Field Data
Main Title Dehydrogenation: A Previously Unreported Pathway of Lindane Metabolism in Mammals.
Author Chadwick, Robert W. ; Chuang, Lucy T. ; Williams., Katherine ;
CORP Author National Environmental Research Center, Research Triangle Park, N.C. Pesticides and Toxic Substances Effects Lab.
Year Published 1975
Report Number found in PB-280 889
Stock Number PB-280 894, found in PB-280 889
Additional Subjects Pesticides ; Toxicology ; Insecticides ; Dehydrogenation ; Mammals ; In vitro analysis ; In vivo analysis ; Metabolism ; Chlorine organic compounds ; DDT ; Assaying ; Physiological effects ; Enzymes ; Liver ; Biochemistry ; Toxic substances ; Reprints ; Lindane ; Metabolites ; Cyclohexene/hexachloro
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB-280 894 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 12p
Abstract
The study presents evidence for the dehydrogenation of lindane by a hepatic microsomal mixed-function oxidase system. Preliminary investigation established that the incubation of lindane with rat liver homogenates produces a chlorinated, nonpolar compound identified as hexachlorocyclohexene. Differential centrifugation resulted in the sedimentation of most of the dehydrogenase activity in the microsomal fraction. Optimum in vitro assay conditions were established and it was found that the dehydrogenase system required molecular oxygen and reduced pyridine nucleotide coenzyme for maximum activity. Inhibition by SKF 525-A and CO suggested that the enzyme was cytochrome P-450 dependent. Lack of inhibition by cyanide indicated that the cytochrome b5 desaturase system was probably not involved. Pretreatment of rats with DDT, which stimulates lindane metabolism, also induced significantly higher dehydrogenase activity. Both the in vivo and in vitro metabolism of hexachlorocyclohexene produced previously identified lindane metabolites. The existence of a cytochrome P-450 dependent mixed-function oxidase which catalyzes the dehydrogenation of lindane has not previously been reported and may be of importance in the metabolism of other xenobiotics.
Notes
contained in PB-280 889 titled Journal Articles on Toxicology. Group 14.