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RECORD NUMBER: 8 OF 1241

OLS Field Name OLS Field Data
Main Title 21-day Feeding Study of Di-n-butyl Phthalate to Rats: Effects on the Liver and Liver Lipids.
CORP Author British Industrial Biological Research Association, Carshalton (England).; Chemical Manufacturers Association, Washington, DC.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 1986
Report Number 40-8626209
Stock Number OTS0509545
Additional Subjects Toxicology ; Health effects ; Phthalate Esters ; Subchronic Toxicity ; Mammals ; Rats ; Oral ; Diet ; Biochemistry ; Toxic substances ; Laboratory animals ; CAS No 84-74-2
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NTIS  OTS0509545 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/2010
Collation 109p
Abstract
Effects on the liver and liver lipids were evaluated in groups of male and female Fischer 344 rats (5/sex/dose level) fed nominal levels of 0, 0.6, 1.2 or 2.5% di-n-butyl phthalate in the diet for 21 days. Toxicity was evident by statistical differences between dosed groups and controls for: mean body weights (2.5% and 1.2% group males & 2.5% group females), food consumption (2.5% group males & females), absolute and relative liver weights (all treated animals), relative kidney weights (1.2 and 2.5% group males & 2.5% group females) and absolute and relative testis weights (2.5% group males). There was a statistically significant decrease in serum cholesterol (all treated animals) and a significant decrease in serum triglycerides (all treated males), although these effects were not considered dose-related. Also observed was an significant increase in serum triglycerides for 2.5% group females. There was a moderate increase in peroxisome proliferation for the high dose animals. Liver biochemistry revealed statistically significant differences between treated and controls as indicated by cyanide-insensitive palmitoyl-CoA oxidation levels (1.2 and 2.5% group males & 2.5% group females), lauric acid 11- and 12- hydroxylase activities (all treated males & 2.5% group females) and total hepatic protein levels (0.6 and 1.2% group males, 1.2 and 2.5% group females). There was no consistent dose response relationship among treatment groups for lipid content in the liver. Histological changes attributable to di-n-butyl phthalate were reduction in cytoplasmic basophilia in the livers of the high dose rats and some of the 1.2% group males. Severe testicular atrophy was observed at the 2.5% dietary level.