Main Title |
Ca(2+)/Calmodulin-Dependent Protein Kinase Enriched in Cerebellar Granule Cells: Identification of a Novel Neuronal Calmodulin-Dependent Protein Kinase. |
Author |
Ohmstede, C. A. ;
Jensen, K. F. ;
Sahyoun, N. E. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Burroughs Wellcome Co., Research Triangle Park, NC. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA/600/J-89/046; |
Stock Number |
PB90-103623 |
Additional Subjects |
Calcium ;
Rats ;
Genetics ;
Chemical analysis ;
Isolation ;
Reprints ;
Protein kinases ;
Calmodulin ;
Brain chemistry ;
Molecular cloning ;
Immunohistochemistry ;
Monoclonal antibodies ;
Poly A ;
Base sequence ;
Immunoblotting
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-103623 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
The authors cloned a novel, brain-specific, Ca/calmodulin-dependent protein kinase from a rat lambda gt11 library which was probed for calmodulin-binding proteins. Antibodies raised against the b-galactosidase fusion protein recognized Mr 65000 and Mr 65000/67000 polypeptides from rat forebrain and cerebellum, respectively. Both polypeptides co-purified to homogeneity and phosphorylated synapsin I. The protein kinase is coded for by two poly A+RNAs at 2.0 kb and 3.5 kb which probably derive from a single gene. A 1.3 kbp cDNA insert contained 477 bp of protein coding sequence which included a calmodulin-binding domain, but no further sequence similarities to other protein kinases. Immunohistochemical localization with a monospecific antibody revealed that the enzyme was expressed in specific subpopulations and was exceptionally enriched in the granule cells of the cerebellum. |