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OLS Field Name OLS Field Data
Main Title Cloning and Characterization of the 'cl' Repressor of 'Pseudomonas aeruginosa' Bacteriophage D3: a Functional Analog of Phage Lambda 'c'I Protein.
Author Miller, R. V. ; Kokjohn, T. A. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Stritch School of Medicine, Maywood, IL. Dept. of Microbiology.
Year Published 1987
Report Number EPA-600/J-87/334;
Stock Number PB88-199419
Additional Subjects Pseudomonas ; Bacteriophages ; Bacterial proteins ; Escherichia coli ; Coliphage ; Reprints ; Repressor proteins ; Phage lambda ; Bacteriophage D3 ; Pseudomonas aeruginosa ; Cloning molecular ; Rec A protein ; Genes ; Bacterial ; Lysogeny
Holdings
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Status
NTIS  PB88-199419 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/04/1988
Collation 9p
Abstract
The authors cloned the gene (c1) which encodes the repressor of vegetative function of Pseudomonas aeruginosa bacteriophage D3. The cloned gene was shown to inhibit plating of D3 and the induction of D3 lysogens by UV irradiation. The efficiency of plating and prophage induction of the heteroimmune P. aeruginosa phage F116L were not affected by the presence of the cloned c1 gene of D3. When the D3 DNA fragment containing c1 was subcloned into pBR322 and introduced into Escherichia coli, it was shown to specifically inhibit the plating of phage lambda and the induction of the lambda prophage by mitomycin C. The plating of lambda imm(434) phage was not affected. (Copyright (c) 1987, American Society for Microbiology.)