Record Display for the EPA National Library Catalog

RECORD NUMBER: 45 OF 105

OLS Field Name OLS Field Data
Main Title Gene Structure in Methanogenic Bacteria.
Author Reeve, J. N. ;
CORP Author Ohio State Univ., Columbus. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;Department of Energy, Washington, DC.
Publisher c1987
Year Published 1987
Report Number EPA-R-810340, DE-AC02-81ER10945; EPA/600/J-87/495;
Stock Number PB90-106279
Additional Subjects Deoxyribonucleic acids ; Biomass ; Escherichia coli ; Bacillus subtilis ; Bacterial genes ; Archaebacteria ; Genetic translation ; Molecular cloning ; Base sequence ; Nucleic acid hybridization
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB90-106279 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 10p
Abstract
Methane-producing microorganisms (methanogens) catalyze the terminal reactions in the anaerobic digestion of biomass. This is a well-established biotechnological process used for both waste treatment and the production of the renewal energy source methane. Although methanogens are procaryotes they are not closely related to well-known eubacterial species, commonly termed bacteria, but are members of the third biological kingdom, the Archaebacteria. The paper presents a brief review of the information currently available documenting the structure of genes in methanogens. A comparison of gene structure in eubacteria, eucaryotes and methanogens is given. Evidence is presented indicating that polypeptide-encoding genes in methanogens are organized into operons and that translation is coordinated, as in eubacteria, by messenger ribonucleic acid:16S ribosomal ribonucleic acid hybridization. Methanogen-derived genes are expressed when cloned in Escherichia coli, which should allow routine genetic engineering procedures to be used to dissect and manipulate the bioprocess of methanogenesis.