Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Enzyme-Linked Immunosorbent Assays for Detection and Quantitation of the Entomocidal Parasporal Crystalline Protein of 'Bacillus thuringiensis' subspp. 'kurstaki' and 'israelensis'.
Author Wie, S. I. ; Andrews, R. E. ; Hammock, B. D. ; Faust, R. M. ; Bulla, L. A. ;
CORP Author California Univ., Davis. ;Kansas State Univ., Manhattan. ;Department of Agriculture, Beltsville, MD.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-R-806447; EPA/600/J-82/469;
Stock Number PB87-115358
Additional Subjects Toxins ; Bacteria ; Reprints ; Immunosorbent assays ; Bacillus thuringiensis
Library Call Number Additional Info Location Last
NTIS  PB87-115358 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 6p
An enzyme-linked immunosorbent assay (ELISA) was used to detect and quantitate the parasporal crystal toxins of Bacillus thuringiensis subspp. kurstaki and israelensis. The ELISA method described is extremely sensitive, accurate, and highly specific. Using the technique, crystalline insecticidal proteins from several subspecies of B. thuringiensis were compared. The dipteran crystal toxin produced by B. thuringiensis subsp. israelensis was shown to share few epitopes with the lepidopteran toxin from subspecies kurstaki, tolworthi, berliner and alesti.