Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Assessment of the Microscreen Phage-Induction Assay for Screening Hazardous Wastes.
Author Houk, V. S. ; DeMarini, D. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Year Published 1987
Report Number EPA/600/D-87/241;
Stock Number PB88-113378
Additional Subjects Genetics ; Toxicity ; Salmonella ; Hazardous materials ; Assaying ; Bacteriophages ; Carcinogens ; Mutagens ; Assessments ; Microscreen phage induction assay ; Hazardous wastes ; Volatile organic compounds
Library Call Number Additional Info Location Last
NTIS  PB88-113378 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 25p
The Microscreen phage-induction assay, which quantitatively measures the induction of prophage lambda in Escherichia coli WP2s(lambda), was used to test 14 crude (unfractionated) hazardous industrial waste samples for genotoxic activity in the presence and absence of metabolic activation. Eleven of the 14 wastes induced prophage, and induction was observed at concentrations as low as 0.4 picograms per ml. Comparisons between the mutagenicity of these waste samples in Salmonella and their ability to induce prophage lambda indicate that the Microscreen phage-induction assay detected genotoxic activity in all but one of the wastes that were mutagenic in Salmonella. Moreover, the Microscreen assay detected as genotoxic 5 additional wastes that were not detected in the Salmonella assay. The applicability of the Microscreen phage-induction assay for screening hazardous wastes for genotoxic activity is discussed along with some of the problems associated with screening highly toxic wastes containing toxic volatile compounds.