||An enclosed aquatic multispecies test system for testing microbial pest control agents with non-target species /
Lightner, Donald V., ;
Thurman, R. B. ;
||Arizona Univ., Tucson. Environmental Research Lab. ;Aquinas Coll., Adelaide (Australia).;Environmental Research Lab., Gulf Breeze, FL.
|| U.S. Environmental Protection Agency, Environmental Research Laboratory,
Biological pest control agents--United States--Testing. ;
Pests--Biological control--United States--Testing. ;
Aquatic animals--United States. ;
Aquatic plants--United States.
Aquatic animals ;
Aquatic plants ;
Aquatic microbiology ;
Histological techniques ;
Water pollution effects(Animals) ;
Bacillus sphaericus ;
Pseudomonas putida ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||61 pages : illustrations ; 28 cm
An enclosed test system was developed in which multiple species of aquatic animals and plants were tested experimentally for adverse non-target effects of wild-type and genetically altered microbial pest control agents (MPCAs). Four different types of model MPCAs were tested in the multispecies system. These included two different strains of the mosquito pathogen Bacillus sphaericus, a strain of Pseudomonas putida (used as a model for the genus), and the insect baculovirus AcMNPV. The fate, persistence, and infectivity of these model MPCAs were evaluated using traditional microbiological and histological methods, as well as specific microbiological assays for model MPCAs that were altered by addition of a unique genetic marker. For two of the model MPCAs, gene probes were used as a detection method to track the MPCA in the test system water and NTOs.
Caption title. "June 1989." "EPA/600/4-89/027." Microfiche.