Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Development of a carcinogen assay system utilizing estuarine fishes
Author Martin, B. J.
CORP Author University of Southern Mississippi, Hattiesburg. Dept. of Biology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher U.S. Environmental Protection Agency, Environmental Research Laboratory ;
Year Published 1983
Report Number EPA-600/3-82-091.
Stock Number PB83-136333
OCLC Number 10236602
Subjects Carcinogenicity testing ; Fishes--Effect of water pollution on ; Biological assay
Additional Subjects Bioassay ; Carcinogens ; Toxicology ; Development ; Lethal dosage ; Fishes ; Estuaries ; Contaminants ; Liver ; Exposure ; Concentration(Composition) ; Anomalies ; Cyprinodon variegatus ; Benzidines ; Cell lines ; Archosargus probatocephalus ; Telecosts ; Toxic substances ; Benzopyrenes
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EKCD  EPA 600-3-82-091 CEMM/GEMMD Library/Gulf Breeze,FL 02/08/2008
NTIS  PB83-136333 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 07/22/2019
Collation x, 50 p. : ill. ; 28 cm.
The objective of this project was the development of systems to assay the effects of chemical carcinogens on marine teleosts. It was determined that the LC-50 for benzidine with respect to Cyprinodon variegatus was ca. 64 ppm. Weekly contaminations of 1 ppm benzidine caused some individuals to develop proliferative liver lesions. Exposure of C. variegatus early embroyos produced the following anomalies at concentrations of 50 ppm and above: tubed heart syndrome with distended pericardia, poor circulation, sparse distribution of melanophores, inability to hatch, abnormal head morphology, scoliosis, and faint RBC pigmentation. Chronic exposure of a cell line from Archosargus probatocephalus to benzidine and benzo(a)pyrene produced mutagenic effects. Two novel techniques were developed to study the effects of carcinogens on C. variegatus at the cellular level -- an aseptic embryo technique and an embryo-primary cell culture technique. Standard immunological techniques were miniaturized to study the immune system of C. variegatus. Serum electrophoresis disclosed that the serum proteins of benzidine-exposed fish differed from unexposed controls.
"November 1982." "EPA-600/3-82-091." Includes bibliographical references.