||Development of a carcinogen assay system utilizing estuarine fishes
Martin, B. J.
||University of Southern Mississippi, Hattiesburg. Dept. of Biology.;Environmental Research Lab., Gulf Breeze, FL.
||U.S. Environmental Protection Agency, Environmental Research Laboratory ;
Carcinogenicity testing ;
Fishes--Effect of water pollution on ;
Lethal dosage ;
Cyprinodon variegatus ;
Cell lines ;
Archosargus probatocephalus ;
Toxic substances ;
||CEMM/GEMMD Library/Gulf Breeze,FL
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||x, 50 p. : ill. ; 28 cm.
The objective of this project was the development of systems to assay the effects of chemical carcinogens on marine teleosts. It was determined that the LC-50 for benzidine with respect to Cyprinodon variegatus was ca. 64 ppm. Weekly contaminations of 1 ppm benzidine caused some individuals to develop proliferative liver lesions. Exposure of C. variegatus early embroyos produced the following anomalies at concentrations of 50 ppm and above: tubed heart syndrome with distended pericardia, poor circulation, sparse distribution of melanophores, inability to hatch, abnormal head morphology, scoliosis, and faint RBC pigmentation. Chronic exposure of a cell line from Archosargus probatocephalus to benzidine and benzo(a)pyrene produced mutagenic effects. Two novel techniques were developed to study the effects of carcinogens on C. variegatus at the cellular level -- an aseptic embryo technique and an embryo-primary cell culture technique. Standard immunological techniques were miniaturized to study the immune system of C. variegatus. Serum electrophoresis disclosed that the serum proteins of benzidine-exposed fish differed from unexposed controls.
"November 1982." "EPA-600/3-82-091." Includes bibliographical references.