Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Mammalian and Avian Transgenesis - New Approaches [electronic resource] /
Author Pease, Shirley.
Other Authors
Author Title of a Work
Lois, Carlos.
Publisher Springer Berlin Heidelberg,
Year Published 2006
Call Number QH432
ISBN 9783540284895
Subjects Life sciences. ; Medicine. ; Cytology. ; Developmental biology. ; Animal genetics.
Internet Access
Description Access URL
Collation XX, 281 p. 49 illus., 12 in color. online resource.
Due to license restrictions, this resource is available to EPA employees and authorized contractors only
Contents Notes
Generation of Transgenic Animals Using Lentiviral Vectors -- Intracytoplasmic Sperm Injection (ICSI) in the Mouse -- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation -- Cloning The Laboratory Mouse by Nuclear Transfer -- Large Insert Transgenesis -- Regional and Temporal Control of Genetic Manipulation in the Mouse -- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes -- Nuclear Transfer in the Cow -- Production of Transgenic Pigs by DNA Microinjection -- Production of Transgenic Birds Using Lentiviral Vectors -- Ancillary Techniques. Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.