Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay.
Author Doern, G. V. ; Herrmann, J. E. ; Henderson, P. ; Stobbs-Walro, D. ; Perron, D. M. ;
CORP Author Massachusetts Univ. Medical Center, Worcester.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA-R-812550; EPA/600/J-86/016;
Stock Number PB86-177813
Additional Subjects Viruses ; Detection ; Humans ; Feces ; Bioassay ; Reprints ; Rotaviruses ; Immunoassay ; Agglutination test
Library Call Number Additional Info Location Last
NTIS  PB86-177813 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 6p
A total of 176 human fecal specimens were examined for the presence of rotavirus using four different assays: a monoclonal antibody enzyme immunoassay; the original polyclonal antibody enzyme immunoassay marketed by Abbott Laboratories, Chicago, IL (Rotazyme I); a modification of this assay which is now commercially available (Rotazyme II); and a latex agglutination test (Rotalex) recently introduced by Medical Technology Corporation, Somerset, NJ. In addition, selected specimens were examined for the presence of rotavirus by electron microscopy, immune electron microscopy and by RNA gel electrophoresis. Forty specimens were positive using the monoclonal antibody enzyme immunoassay; 136 were negative. Using the results obtained with the procedure as the reference standard, the sensitivities of the Rotazyme I, Rotazyme II and Rotalex tests were 97.4, 100, and 81.6% respectively. The specificities of these three procedures were 88.8, 83.9, and 100%, respectively.