Record Display for the EPA National Library Catalog

RECORD NUMBER: 687 OF 1214

OLS Field Name OLS Field Data
Main Title Methods for Measuring Mutagenicity in Urine of Rats Dosed with (14C)di(2-ethylhexyl) Phthalate with Cover Letter.
CORP Author Eastman Kodak Co., Rochester, NY.;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Publisher 3 Feb 1984
Year Published 1984
Report Number OTS-878213940;
Stock Number OTS-0206391
Additional Subjects Mutagenic pathways ; Urine ; Rats ; Doses ; Solvents ; Phthalates ; Toxicity ; Salmonella ; In vitro analysis ; Absorption ; Microsomes ; Adsorption ; Metabolites ; Di(2-Ethylhexyl)phthalate ; Alkyl phthalates
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NTIS  OTS-0206391 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/29/2008
Collation 112p
Abstract
Di (2-ethylbexyl) phthalate (DEHP) is extensively used as a plasticizer for vinyl plastic articles. It has been found to be positive in an NCI rodent bioassay but has generally given negative results in vitro genotoxicity tests. We therefore decided to test the urine of rats fed (14 C) DEHP for mutagenic activity in the Ames Salmonella test. The recovery of radioactivity from the urine of rats dosed with (14 C) DEHP was examined by solvent extraction and XAD-2 resin absorption procedures. Both of these procedures were inadequate for quantitative recovery of urinary metabolites required for subsequent mutagenicity testing using the Ames Salmonella/microsome procedure. Recoveries of less than five percent were observed using standard solvent extraction techniques whereas the XAD-2 adsorption technique gave about 67 percent at high resin/urine ratios.