Record Display for the EPA National Library Catalog
RECORD NUMBER: 234 OF 307
|OLS Field Name||OLS Field Data|
|Main Title||Reoviruses in water pollution testing : final report /|
|Author||Spendlove, Rex S. ; Barnett, B. B. ; George, D. B. ; Adams, D. J. ; Ridinger, D. N.|
|CORP Author||Utah State Univ., Logan.;Health Effects Research Lab., Research Triangle Park, NC.|
|Publisher||U.S. Environmental Protection Agency,|
|Report Number||EPA/600/1-84/022; EPA-R-806527|
|Subjects||Reoviruses. ; Sewage.|
|Additional Subjects||Reoviruses ; Water pollution ; Tests ; Sewage ; Enzymes ; Recovery ; Bioassay ; Tolerances(Physiology) ; Protamines ; Precipitation(Chemistry) ; Evaluation ; Abundance ; Safety ; Rotoviruses|
|Collation||59 pages ; 28 cm|
Twelve cell lines were tested to determine their sensitivity to reoviruses of three serotypes that had been isolated from sewage. Madin-Darby bovine kidney (MDBK) cells were the most susceptible. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and plaque assay procedures. Immunofluorescence assay is more sensitive and more rapid than plaque assay procedure for quantifying reoviruses recovered from sewage. A procedure for protamine sulfate precipitation of reoviruses and rotaviruses was developed. Some reoviruses recovered from sewage were inactivated by enzyme treatment. This report describes a study to evaluate reoviruses for their potential usefulness in water quality testing.
Caption title. "Final Report." "Nov. 1984." "EPA-600/1-84-022." Microfiche.