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OLS Field Name OLS Field Data
Main Title Recruitment of a Chromosomally Encoded Maleylacetate Reductase for Degradation of 2,4-Dichlorophenoxyacetic Acid by Plasmid pJP4.
Author Kukor, J. J. ; Olsen, R. H. ; Siak, J. S. ;
CORP Author Michigan Univ., Ann Arbor. Medical School. ;General Motors Research Labs., Warren, MI.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1989
Year Published 1989
Report Number EPA-R-812679; EPA/600/J-89/436;
Stock Number PB90-264763
Additional Subjects Chromosomes ; Pseudomonas ; Biodeterioration ; Deoxyribonucleic acids ; Reprints ; Maleylacetate reductase ; 2-4 dichlorophenoxyacetic acid ; Plasmids ; Molecular cloning ; Gene expression regulation ; Polyacrylamide gel electrophoresis
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NTIS  PB90-264763 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/03/1990
Collation 8p
Abstract
When Pseudomonas aeruginosa PA01c or P. putida PP0220 or PP0300 carry plasmid pJP4, which encodes enzymes for the degradation of 2,4-dichlorophenoxyacetic acid (TFD) or 2-chloromaleylacetate, cells do not grow on TFD and UV-absorbing material with spectral characteristics of chloromaleylacetate accumulates in the culture medium. Using plasmid pR01727, DNA fragments which contain the gene for maleylacetate reductase, were cloned from the chromosome of a nonfluorescent pseudomonad, Pseudomonas sp. strain PK01, 6- and 0.5-kilobase BamHI. A novel polypeptide with an estimated molecular weight of 18,000 was detected in cell extracts of P. aeruginosa carrying either plasmid pR01944 or plasmid pR0945. Maleylacetate reductase activity was induced in cells of P putida carrying plasmid pR01945, as well as in cells in Pseudomonas strain PK01, when grown on L-tyrosine, suggesting that the tyrosine catabolic pathway might be the source from which maleylacetate reductase is recruited for the degradation of TFD in pJP4-bearing cells of Pseudomonas sp. strain PK01. (Copyright (c) 1989 American Society for Microbiology.)