Record Display for the EPA National Library Catalog

RECORD NUMBER: 49 OF 92

OLS Field Name OLS Field Data
Main Title Level 1 Bioassay Sensitivity.
Author Brusick, D. J. ; Young, R. R. ;
CORP Author Litton Bionetics, Inc., Kensington, MD.;Industrial Environmental Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-58-02-2681; EPA-600/7-81-135;
Stock Number PB82-221201
Additional Subjects Environmental surveys ; Bioassay ; In vitro analysis ; In vivo analysis ; Assessments ; Mutagens ; Samples ; Toxicity ; Tables(Data) ; Concentration(Composition) ; Industrial hygiene ; Toxic substances
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB82-221201 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 59p
Abstract
The report, based on a survey of published literature, establishes a set of sensitivity estimates for the Level 1 environmental assessment (EA) bioassays recommended by EPA's IERL-RTP. The estimates permit comparison of bioassay sensitivity for detecting toxicity or mutagenicity of pure chemicals and also estimation of the concentrations of toxic materials that could be in a mixture that registers negative in a particular test. Three tests--Ames Salmonella/microsome mutagenesis assay, in vitro rodent cell (CHO) clonal toxicity assay, and in vivo rodent toxicity assay--have substantial published data bases using study designs similar to those used in Level 1 EA tests. The report summarizes the sensitivity limits for detecting toxicity or mutagenicity for a variety of pure compounds with these three tests. Methods developed to assess these data will be applicable to other Level 1 tests such as the rabbit alveolar macrophage (RAM) assay and the aquatic and other ecological assays. However, these evaluations await the development of a sufficient data base with a wide variety of pure compounds. Two of the reviewed bioassays (Ames and CHO) were found to approach the sensitivity required of the Level 1 chemical analytical procedures.