||Studies of Echovirus-12 in Volunteers: Determination of Minimal Infectious Dose and the Effect of Previous Infection on Infectious Dose.
Schiff, G. M. ;
Stefanovic, G. M. ;
Young, E. C. ;
Sander, D. S. ;
Pennekamp, J. K. ;
||Christ Hospital, Cincinnati, OH. Inst. of Medical Research.;Health Effects Research Lab., Research Triangle Park, NC.
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A two-part study of echovirus-12 was done in volunteers. In the first part the human infectious dose of the virus was determined in 149 healthy adults with undetectable serum antibody, each of whom drank 0-330,000 plaque-forming units (pfu) of virus in 100 ml of nonchlorinated water. Infection was defined as fecal shedding of virus or significant (fourfold or greater) increases in serum antibody titer. The HID(50) (i.e., the dose required for infection of 50% of the volunteers) was 919 pfu. Through statistical analysis of the data by probit transformation, a 1% human-infectious dose of 17 pfu was predicted.