Record Display for the EPA National Library Catalog

RECORD NUMBER: 26 OF 30

OLS Field Name OLS Field Data
Main Title Specific Proteins and Their Application in Wastewater Treatment.
Author Furlong, C. E. ; Sundstrom, J. A. ; Weiler, E. B. ; Cheung, P. W. ; Yin, J. ;
CORP Author Washington Univ., Seattle. ;California Univ., Berkeley. Dept. of Chemical Engineering.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher Oct 88
Year Published 1988
Report Number EPA-R-811948; EPA/600/D-88/228;
Stock Number PB89-119721
Additional Subjects Water pollution abatement ; Escherichia coli ; Proteins ; Cadmium ; Phosphates ; Polymers ; Peptides ; Waste water treatment ; Bioreactors ; Carrier proteins ; Metallothionein
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB89-119721 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/14/1989
Collation 33p
Abstract
The purpose of the research was to test the feasibility of using immobilized proteins as highly specific adsorbers of pollutants in waste streams. The Escherichia coli periplasmic phosphate-binding protein served as both a model system for determining the feasibility of such an approach and at the same time was used to produce a cycling ligand adsorber capable of efficiently scrubbing phosphate from a feed stream. Three different procedures for the removal of cadmium from feed streams were developed in this project. A bioreactor for continuous protein production was developed to minimize the cost of protein production. The bioreactor contained immobilized, non-growing E. coli cells. The cell line had been mutagenized and selected for the direct secretion of periplasmic proteins into the medium. The cells contained the gene that encodes the phosphate-binding protein.