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RECORD NUMBER: 4 OF 4

OLS Field Name OLS Field Data
Main Title Primary Structure of the P450 Lanosterol Demethylase Gene from 'Saccharomyces cerevisiae'.
Author Kalb, V. F. ; Woods, C. W. ; Turi, T. G. ; Dey, C. R. ; Sutter, T. S. ;
CORP Author Cincinnati Univ., OH. Dept. of Microbiological and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1993
Year Published 1993
Report Number EPA-R-810605; EPA/600/J-93/302;
Stock Number PB93-229243
Additional Subjects Cytochrome P-450 ; Saccharomyces cerevisiae ; Fungal structural genes ; Fungal DNA ; Molecular sequence data ; Open reading frames ; Plasmids ; Restriction mapping ; Genetic transcription ; Amino acid sequence homology ; Reiterated genes ; Reprints ;
Holdings
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NTIS  PB93-229243 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 11p
Abstract
The structural gene and flanking regions for lanosterol 14alpha-demethylase (14DM) from Saccharomyces cerevisiae has been sequenced. An open reading frame of 530 codons encodes a 60.7-kDa protein. When this gene is disrupted by integrative transformation, the resulting strain requires ergosterol and, as expected, grows only in the absence of oxygen. The deduced amino acid sequence of 14DM includes a hydrophobic segment near the amino terminus which may be a transmembrane domain. The deduced sequence has been compared with those of eight other eukaryotic P450s, each from a different family within the P450 superfamily. These comparisons indicate that this yeast P450 and several mammalian P450s have equivalent alignment scores when each is compared with the bovine P450scc. Matrix comparisons of the amino acid sequence of this P450 with those of mammalian P450s reveal three conserved regions. The DNA region 5' to the structural 14DM gene includes poly(dA:dT) sequences and a repeating hexamer sequence.