Record Display for the EPA National Library Catalog

RECORD NUMBER: 9 OF 49

OLS Field Name OLS Field Data
Main Title Cutworm Peridroma saucia (Lepidoptera: Noctuidae) Supports Growth and Transport of pBR322-Bearing Bacteria.
Author Armstrong, J. L. ; Porteous, L. A. ; Wood, N. D. ;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/244;
Stock Number PB90-137035
Additional Subjects Bacteria ; Klebsiella ; Enterobacter ; Erwinia ; Plants(Botany) ; Larvae ; Reprints ; Plasmids ; Peridroma saucia ; Microcosms ; Cutworms
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-137035 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/15/1990
Collation 8p
Abstract
Variegated cutworms were exposed to bean plants in microcosms sprayed with pBR322-carrying strains of Enterobacter cloacae, Klebsiella planticola, and Erwinia herbicola. The three bacterial species exhibited differential survival on leaves, in soil, and in guts and fecal pellets (frass) of the insects. High numbers of Enterobacter cloacae (pBR322) were detected in all samples, while the other species were unable to establish residence in the insect. To assess the impact of this colonization on site-to-site transport of microorganisms, larvae were fed plants that had been sprayed with the bacteria and then were transferred to uninoculated plants. Cutworms were efficient carriers of Enterobacter cloacae (pBR322), as indicated by its rapid appearance on uninoculated leaves and continued persistence in the insects for 3 days after transfer. Few Erwinia herbicola (pBR322) and K. planticola (pBR322) were obtained from larvae after transfer, although up to 10(3) CFU/g were detected in soil and on plants. Differences in bacterial survival and growth were confirmed by incubating frass overnight and observing the change in population numbers. The proportion of total samples showing at least a 25-fold increase during incubation was 68% for Enterobacter cloacae (pBR322), 39% for K. planticola (pBR322), and 0% for Erwinia herbicola (pBR322).