Record Display for the EPA National Library Catalog

RECORD NUMBER: 35 OF 79

OLS Field Name OLS Field Data
Main Title Effect of Incubation and Activation Conditions on the Hepatocyte-Mediated Plate Incorporation and Preincubation 'Salmonella typhimurium' Mutagenesis Assays.
Author Williams, K. ; Inmon, J. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-600/J-83-137;
Stock Number PB84-144336
Additional Subjects Bacteria ; Reprints ; Salmonella typhimurium ; Mutagenesis ; Cell mediated assays
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB84-144336 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 12p
Abstract
Primary cell-mediated microbial mutagenesis assays have been shown to be useful in detecting specific target organ genotoxic activity. The lack of a standard protocol for these assays, however, makes interlaboratory comparisons difficult. In order to standardize the hepatocyte-mediated Salmonella typhimurium mutagenesis assay, incubation and activation conditions for the plate incorporation and preincubation assays were examined using two aromatic amines, 2-aminofluorene (AF) and 2-acetylaminofluorene (AAF). Direct comparison of two preincubation protocols demonstrated the necessity for the hepatocytes to be present during the two-to three-day plate incubation period.