||Effect of Incubation and Activation Conditions on the Hepatocyte-Mediated Plate Incorporation and Preincubation 'Salmonella typhimurium' Mutagenesis Assays.
Williams, K. ;
Inmon, J. ;
Lewtas, J. ;
||Health Effects Research Lab., Research Triangle Park, NC.
Salmonella typhimurium ;
Cell mediated assays
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Primary cell-mediated microbial mutagenesis assays have been shown to be useful in detecting specific target organ genotoxic activity. The lack of a standard protocol for these assays, however, makes interlaboratory comparisons difficult. In order to standardize the hepatocyte-mediated Salmonella typhimurium mutagenesis assay, incubation and activation conditions for the plate incorporation and preincubation assays were examined using two aromatic amines, 2-aminofluorene (AF) and 2-acetylaminofluorene (AAF). Direct comparison of two preincubation protocols demonstrated the necessity for the hepatocytes to be present during the two-to three-day plate incubation period.