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RECORD NUMBER: 34 OF 79

OLS Field Name OLS Field Data
Main Title Effect of Incubation and Activation Conditions on the Hepatocyte-Mediated Plate Incorporation and Preincubation 'Salmonella typhimurium' Mutagenesis Assays.
Author Williams, K. ; Inmon, J. ; Lewtas, J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA/600/J-83/339;
Stock Number PB86-199486
Additional Subjects Hepatocytes ; Toxicology ; Reprints ; Mutagenesis ; Salmonella typhimurium ; Aminofluorene ; Acetylaminofluorene
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB86-199486 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 12p
Abstract
Primary cell-mediated microbial mutagenesis assays have been shown to be useful in detecting specific target organ genotoxic activity. The lack of a standard protocol for these assays, however, makes interlaboratory comparisons difficult. In order to standardize the hepatocyte-mediated Salmonella typhimurium mutagenesis assay, incubation and activation conditions for the plate incorporation and preincubation assays were examined using two aromatic amines, 2-aminofluorene (AF) and 2-acetylaminofluorene (AAF). Direct comparison of two preincubation protocols demonstrated the necessity for the hepatocytes to be present during the two- to three-day plate incubation period. An examination of various preincubation times showed relatively minor differences between 15 and 90 minutes. The preincubation and plate incorporation protocols were directly compared using both hamster and rat hepatocytes.