||Tissue Culture as a Method for Evaluating the Biotransformation of Xenobiotics by Plants.
Wickliff, C. ;
Fletcher., J. S. ;
||Corvallis Environmental Research Lab., OR. ;Oklahoma Univ., Norman.
Tissue culture ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Suspension cultures of Rosa cv. Paul's scarlet were used as a model system to examine the metabolism of 1,3-dinitrobenzene (DNB), an industrial waste compound. In a three-day period, 90% of the DNB supplied (96 nmoles) was metabolized by approximately 12 grams (fresh weight) of cells. The primary end product of DNB metabolism partitioned into the insoluble residue fraction of the extracted cells. The study demonstrates the usefulness of plant tissue cultures in evaluating the chemical influence of plants on xenobiotics.