||Polymeric Beta-Hydroxyalkanoates from Environmental Samples and 'Bacillus megaterium'.
Findlay, Robert H. ;
White, David C. ;
||Florida State Univ., Tallahassee.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.;National Oceanic and Atmospheric Administration, Rockville, MD. Office of Sea Grant.
||EPA-R-807321 ;NSF-OCE80-19757; EPA-600/J-83-016;
Chemical analysis ;
Oxygen organic compounds ;
Gas chromatography ;
Fatty acids ;
Poly(butyric acid/(hydroxy-ester)) ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The procaryotic endogenous storage polymer known as poly-beta-hydroxybutyrate is actually a mixed polymer of short-chain beta-hydroxy fatty acids. A method for the quantitative recovery of this mixed polymer, called poly-beta-hydroxyalkanoate (PHA), with analysis by capillary gas-liquid chromatography, showed the presence of at least 11 short-chain beta-hydroxy acids in polymers extracted from marine sediments. Polymers extracted from Bacillus megaterium monocultures were also a complex mixture of beta-hydroxy acids with chain lengths between four and eight carbons. Lyophilized sediments were extracted in a modified Soxhlet extractor, and the polymer was purified with ethanol and diethyl ether washes. The purified polymer was treated with ethanol-chloroform-hydrochloric acid (8.5:2.5:1) for 4 h at 100 C, a treatment which resulted in the formation of the ethyl esters of the constituent beta-hydroxy acids.