Record Display for the EPA National Library Catalog

RECORD NUMBER: 22 OF 74

OLS Field Name OLS Field Data
Main Title Energy Dispersive X-ray Microanalysis of Vital Dye (Erythrosin B) Stained Cells.
Author Walker, S. R. ; Ingram, P. ; Shelburne, J. D. ;
CORP Author Duke Univ. Medical Center, Durham, NC. ;Veterans Administration Medical Center, Durham, NC. ;Research Triangle Inst., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1984
Report Number EPA-R-807560; EPA/600/J-84/353;
Stock Number PB86-136207
Additional Subjects Dyes ; X-rays ; Cells(Biology) ; Microscopy ; Energy ; Reprints ; Erythrosin
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB86-136207 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 4p
Abstract
One serious problem encountered during microprobe studies of freeze-dried frozen sections is that it is difficult or impossible to identify healthy versus injured cells using morphologic criteria. There is a need for an independent method to identify cells which have lost plasma membrane integrity. Vital dyes such as trypan blue and erythrosin B have been used for many years in light microscopic studies of the viability of cultured cells. It is generally accepted that cells which stain positively (i.e., take up the dye) have lost plasma membrane integrity and are lethally injured. Since erythrosin B is iodinated, the authors were able to show that lethally injured cells were iodine positive, and that iodine positive cultured cells would show evidence of some of the ion shifts that are thought to occur in severe cell injury.